Synthesis of Aflatoxin G1 Artificial Antigen

Abstract

Abstract: Peracid m-chloroperbenzoic acid as the oxidant, aflatoxin G1 was transformed into AFG1-8,9-epoxide,which was coupled to BSA in a two-phase system. The UV spectrum of the conjugate AFG1-BSA is different from BSA and Aflatoxin G1 obviously, and its fluorescence intensity is different from BSA. All these showed that the preparation of AFG1-BSA is successful. The molar ratio of AFG1 to BSA in adduct is 4.29:1. BALB/c mice were immunized with AFG1-BSA for 6 weeks, and the titer antiserum from those mice were testified by indirect ELISA, as 1:16000. The sensitivity of the antibody and its cross-reactivity with AFB1-OVA were also assayed.

Key words: aflatoxin G1, artificial antigen, ELISA

ZHANG Jin-Yang, LI Pei-Wu, ZHANG Wen, DING Xiao-Xia, MA Liang, WU Wen-Hua, ZHANG Xian-Zhong, LI Tao. Synthesis of Aflatoxin G1 Artificial Antigen[J]. FOOD SCIENCE, 2008, 29(6): 194-197.

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Synthesis of Aflatoxin G1 Artificial Antigen

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