Application of Response Surface Methodology for Extraction Optimization of Water-soluble Polysaccharides from Pteridium aquilinum

Abstract

Abstract: The optimum conditions for the extraction of the water-soluble polysaccharides from Pteridium aquilinum were determined using response surface methodology (RSM). A central-composite experimental design (CCD) was used to investi-gate the interaction effects of three independent variables, namely liquid-solid ratio, extraction temperature and extraction time. Also, the antioxidant activity of the crude polysaccharides obtained from Pteridium aquilinum was investigated in this study. The optimum conditions are as follows: liquid-solid ratio 18.8:1 (V/W), extraction temperature 62.5 ℃ and extraction time 5.9 h. The crude polysaccharide shows excellent antioxidant activity according to FARP antioxidative assay.

Key words: response surface methodology, polysaccharides, antioxidatnt activity, Pteridium aquilinum

XU Wen-Tao, ZHANG Fang-Fang, HUANG Kun-Lun, YUAN Yan-Fang, WANG Ying, LUO Yun-Bo. Application of Response Surface Methodology for Extraction Optimization of Water-soluble Polysaccharides from Pteridium aquilinum[J]. FOOD SCIENCE, 2008, 29(7): 122-126.

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Application of Response Surface Methodology for Extraction Optimization of Water-soluble Polysaccharides from Pteridium aquilinum

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