FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 422-424.

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Study on Conditions Optimization of Shake Flask Fermentation for Producing β-glycosidase

 ZHU  Feng-Mei, DU  Bin, LIU  Chang-Jiang, LI  Jun   

  1. 1. College Food Science,Shenyang Agricultural University,Shenyang 110161,China;2.Deportment of Food Engineering,Hebei Normal University of Science and Technology,Changli 066600,China;3.Analysis and Testing Center,Hebei Normal University of Science and Technology,Changli 066600,China
  • Online:2008-08-15 Published:2011-08-26

Abstract: In this study,the shake flask fermentation condition for producingβ-glycosidase by fusion strains of Aspergillus niger and Aspergillus oryzae were optimized by orthogonal test. The results showed that the optimum conditions are rotational rate 180 r/min,fermentation temperature 27 ℃,inoculation amount 12%,medium volume 20 ml/250-ml shake flask and fermentation period 7 days. By these,the activity of β-glucosidase reaches 5.312 U/ml.

Key words: &beta, -glycosidase; shake flask fermentation condition; optimization