Abstract: A multi-residue method was developed for the confirmation and quantitation of nineteen kinds of quinolones in animal food by HPLC-ESI-MS/MS. The samples were extracted with acetonitrile. The analytes were separated using 0.1% fomic acidmethanol system as the mobile phase with a linear gradient elution program,and determined by tandem mass detector. Results showed that their linear ranges are 0.3μg/kg to 50 μg/kg with correlation coefficients(r) more than 0.9956,and the limits of detection are 0.3 μg/kg. The mean recoveries for each analyte in chicken and fish range from 75.3%～96.3% and 79.7%～94.2% with relative standard deviations below 10%. The method is convenient,fast,safe,sensitive and accurate,and can be used to simultaneously analyze residual quinolones in actual samples.

Key words: quinolones, animal food, HPLC-ESI-MS/MS, multi-residue