Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method

Abstract

Abstract: Two kinds of 3,5-dinitrosalicylic acid(DNS) agents were respectively used for determination of reducing sugar content,and effects on determination results by the factors,such as amount of DNS reagent developing time,wavelength and storage time were discussed. The results showed that on the conditions of reagent amount around 1.5 ml,boiling bath time not less than 5 minutes,and standing for 30 minutes after developing,at the wavelength of 540 nm and 550 nm for reagent one and two respectively,the accurate results can be acquired.

Key words: 3,5-dinitrosalicylic acid(DNS), reducing sugar, spectrophotometry, determination factor

ZHAO Kai, XU Peng-Ju, GU Guang-Ye. Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method[J]. FOOD SCIENCE, 2008, 29(8): 534-536 https://doi.org/

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Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method

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