Abstract:

A total of 40 Kunming mice were randomly divided into 4 groups (group A, group B, group C and group D), with half male and half female. Group A as the control was subject to oral administration and then intraperitoneal injection of normal saline. Group B as the oxidative damage model, group C and group D all were subject to intraperitoneal injection of cyclophosphamide and then oral administration of normal saline, and low dose and high dose of squid ink extract, respectively. The organ index of spleen, content of MDA and the activities of SOD, CAT and GSH-Px in spleen tissue were determined, and the quantity of leukocytes in peripheral blood was counted. Compared to the control group, cyclophosphamide was able to decrease the organ indexes of spleen (p ＜ 0.05), as well as the SOD, CAT and GSH-PX activities (p ＜ 0.01) and increase MDA level in spleen tissue. Compared to the oxidative damage model group, high and low doses of squid ink extract could improve the above oxidative damage symptoms at different degrees, but the therapeutic effect of the former was not more apparent than that of the latter was. In conclusion, sepia ink extract at the suitable concentration is able to alleviate the in vivo immunosuppression induced by cyclophosphamide in mice.

Key words: sepia ink, oxidative damage, immunosuppression, spleen