Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate

doi:10.7506/spkx1002-6630-200918074

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 321-322.doi: 10.7506/spkx1002-6630-200918074

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Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate

ZHANG Yan1,2，LU Pin3，WANG Hong2，ZHANG Jing-xuan2，LI Hui2，LIU Jing-ze1,*

1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China；2. Hebei Key Laboratory of Food Safety,
Shijiazhuang 050051, China；3. School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China

Received:2009-06-30 Online:2009-09-15 Published:2010-12-29
Contact: LIU Jing-ze E-mail:liujingze@malil.hebtu.edu.cn

Abstract

Abstract:

A HPLC method was developed for the simultaneous determination of cAMP and cGMP in commerical jujube juice concentrate. Both concentrations of cAMP and cGMP exhibited a good linear relationship with peak area in the range of 0－50 mg/L. The limits of detection (LOD) of the method to them were both 0.1 mg/kg. The spiked recoveries at three levels (1, 10 and 20 mg/L) ranged from 82.2% to 99.3%, with a RSD between 2.1% and 10.9%. The method is suitable for the effective determination of cAMP and cGMP in jujube juice concentrate.

Key words: HPLC, jujube juice concentrate, cyclic nucleotides, detection method

CLC Number:

R155.5

ZHANG Yan1,2，LU Pin3，WANG Hong2，ZHANG Jing-xuan2，LI Hui2，LIU Jing-ze1,*. Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate[J]. FOOD SCIENCE, 2009, 30(18 ): 321-322 https://doi.org/10.7506/spkx1002-6630-200918074

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Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate

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