FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 114-117.doi: 10.7506/spkx1002-6630-200924022

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Separation of Oligopeptides from Deer Placenta via Ultrafiltration

PI Yu-zhen,CAO Xiao-yu,WANG Shu-qin   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, Chi
  • Received:2009-07-11 Online:2009-12-15 Published:2010-12-29
  • Contact: PI Yu-zhen,


Active oligopeptides are the most effective ingredients in deer placenta. The oligopeptides was separated through ultrafiltration from deer placenta. The major factors such as material-liquid ratio, pressure, temperature, flow rate, condensation folds and operation time were investigated to evaluate the effect on separation efficiency. The optimal separation condition was 1.5% of material-liquid ratio, 0.06 MPa of pressure, 30 ℃ of temperature, 2.0 ml/s of flow rate, 2.5 folds for condensation degree and 3 min of operation time. Protein content in separated samples was 56.22% under this optimal condition. The contaminated membrane was cleaned using 0.2% NaOH for 2 h of soaking and 1 h of circulation first, then using HCl for 1 h of soaking and 0.5 h of circulation, and finally using water washing until neutrality. An excellent recovery rate for membrane flux was achieved and WFR value was 0.834.

Key words: deer placenta oligopeptide, ultrafiltration, membrane contamination

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