Abstract:

Two methods, i.e., ultrasonic assisted extraction and cold soaking extraction method, for extraction of mulberroside A from mulberry root cortex were compared, and the effects of ethanol concentration, ratio of raw material to ethanol, soak time or extraction time on the yield of mulberroside A were studied. An orthogonal test was employed to optimize the ultrasonic assisted extraction process. Results suggested that the optimum conditions were that mulberry root cortex was extracted with anhydrous ethanol for 45 min after cold soaking for 1 h, with the ratio of raw material to ethanol at 1:30 (g/ml). Under such conditions, the yield of mulberroside A was 1.734%.

Key words: mulberroside A, ultrasound, extraction