Abstract:

The contents of 7 water-soluble vitamins in fruit powders of Nitraria, wolfberry and weabuckthorn grown in Qinghai- Tibetan Plateau were determined by high performance liquid chromatography (HPLC). Results showed that the contents of 7 water-soluble vitamins were successfully determined simultaneously by sequential treatment of extraction followed by chromatographic separation and the recoveries for spiked standards and the relative standard deviations were 88.0%－113.4% and 1.1% － 9.0%, respectively. Significant differences in the contents of VC, VB1 and VB2 were observed among the three fruit powders. The contents of VC and VB1 were decreased in the following order: seabuckthorn＞ Nitraria ＞ wolfberry, and wolfberry ＞ Nitraria ＞ seabuckthorn, respectively. However, higher VB2 was exhibited in Nitraria fruit powder than in wolfberry powder, whereas no VB2 was detected in seabuckthorn powder. Moreover, fruit powders of seabuckthorn and Nitraria were abundant in VC with contents of (18266.4 ± 201.1) mg/kg and (1468.5 ± 24.6) mg/kg, respectively. In additions, no other water-soluble vitamins like nicotinic acid, pyridoxine hydrochloride, folic acid and vitamin B12 were also detected in the three fruit powders.

Key words: Nitraria, wolfberry, seabuckthorn, fruit powder, water-soluble vitamins, HPLC