Abstract:

Objective: To evaluate the effect of Stropharia rugoso-annulata extract (SRE) on antioxidative enzyme activity and isozyme profile in liver and other major visceral organs of mice with CCl4-induced acute liver injury. Methods: After 4 days of normal feeding, CIR female mice (25 ± 2) g were used as experimental animals. Ten CIR mice orally administered distilled water were served as control group. Fifty-six more CIR mice were randomly divided into 4 groups of 14 mice each: SRE groups orally administered SRE solution in distilled water at low (100 mg/(kg bw·d)), medium (200 mg/(kg bw·d)) and high (400 mg/(kg bw·d)) doses and model group orally administered distilled water. All mice were administered for 30 consecutive days and low-, medium- and high-dose SRE groups and model group were fed water fasting diet for 16 h on the 30th day before oral administration of CCl4 dispersed in salad oil for creating CCl4-induced acute liver injury model. At 24 h postadministration, all mice were sacrificed to assay activities of antioxidant enzymes in liver, heart, kidney and spleen and analyze isozyme profile of CAT and SOD by gradient polyacrylamide gel electrophoresis. Results: Compared with the model group, high-dose SRE group exhibited significantly increased activities of total SOD, Mn-SOD, CuZn-SOD, GPx and CAT in liver; In addition, there was a significant increase of activities of SOD, CAT and GPx in heart and kidney in three SRE groups. Compared with the normal group, the model group exhibited a compensatory increase of Mn-SOD in kidney; however, Mn-SOD activity was decreased to the normal group level after SRE administration. One CAT band was detected in liver, kidney and heart and CCl4-induced acute liver injury resulted in a significant decrease of staining intensity of the brand. In contrast, the staining intensity of the band was increased to the normal group level after SRE administration. Similarly, one Mn-SOD and three CuZn-SOD bands were detected in liver and the staining intensity of the isozymes was also increased to the normal group level after SRE administration. One Mn-SOD and four CuZn-SOD bands were detected in heart and kidney and SRE administration resulted in the increase of staining intensity of CuZn-SOD bands. Conclusion: SRE can protect liver and other major visceral organs of mice with CCl4-induced acute oxidative injury by enhancing the activities of antioxidant enzymes and the contents of isozymes.

Key words: Stropharia rugoso-annulata extract, acute liver injury, antioxidant enzyme, isozyme, gradient polyacrylamide gel electrophoresis