Abstract: In order to provide experimental references for solving the problem of quality deterioration during the storage and fresh-keeping Capsella bursa-pastoris (L.) Medicus, the major phenols in the edible wild vegetable were extracted by grinding in the presence of methanol and analyzed by UV-visible spectrophotometry and high performance liquid chromatography (HPLC). Besides, crude peroxidase (POD) extracted from the edible wild vegetable was measured for its substrate specificity. The results showed that the maximum absorption wavelength of phenolic extract from Capsella bursa-pastoris (L.) Medicus was 213 nm. The major phenolic component was initially identified as pyrogallic acid. The preferred phenolic substrate of POD was identified as chorogenic acid with the optimal enzymatic reaction concentration of 8 mmol/L.

Key words: Capsella bursa-pastoris (L.) Medicus, polyphenol, peroxidase, substrate specificity