Antibacterial Mechanism of Lipopeptide from Bacillus natto against Vibrio parahemolyticus

Abstract

Abstract: Objective：To investigate the antibacterial mechanism of lipopeptide from Bacillus natto against Vibrio parahemolyticus. Method: The effects of antibacterial lipopeptide from Bacillus natto on the growth, cell ultrastructure, cell membrane permeability, protein synthesis, and respiratory metabolism of Vibrio parahemolyticus were evaluated. Results: The antibacterial lipopeptide increased cell membrane permeability of Vibrio parahemolyticus, causing intracellular macromolecules with ultraviolet absorption to leak out of the cells. Under scanning electron microscope, it was observed that the cell wall of Vibrio parahemolyticus was ruptured by the antibacterial lipopeptide and holes were formed. The antibacterial lipopeptide could be bound to DNA from Vibrio parahemolyticus in vitro, resulting in slight blue shift of DNA maximum absorption and hyperchromic effect. SDS-PAGE suggested that the antibacterial lipopeptide had no apparent effect on protein synthesis in Vibrio parahemolyticus cells. The respiratory metabolism of Vibrio parahemolyticus was strongly inhibited by the antibacterial lipopeptide when its concentration was higher than 0.6 MIC. Conclusion: The antibacterial lipopeptide from Bacillus natto increases cell membrane permeability of Vibrio parahemolyticus, causing intracellular materials to leak out of the cells and consequently resulting in growth inhibition and even death of the cell.

Key words: antibacterial lipopeptide, Bacillus natto, Vibrio parahemolyticus, antibacterial mechanism

CLC Number:

References

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