Isolation and Purification of Key Tartaric Acid Degrading Enzyme from Aspergillus niger F1

Abstract

Abstract: The purpose of this study was to prepare a key tartaric acid degrading enzyme from Aspergillus niger F1. Aspergillus niger F1 was cultured and the expression of tartaric acid degrading enzymes was induced by adding tartaric acid into the medium. Cultured mycelia of Aspergillus niger F1 were collected and homogenized in liquid nitrogen. Crude enzyme extract was obtained by ultrasonic assisted extraction, added with protamine sulfate for removing nucleic acids, denatured for removing unwanted proteins, concentrated by PEG treatment, dialyzed for desalting, and then purified by DEAE-cellulose 52 anion exchange chromatography and Sephadex G-75 gel filtration chromatography to obtain a single component with a purification factor of 14.32. The enzyme was proven to be a dehydrogenase.

Key words: Aspergillus niger, tartaric acid degrading enzymes, separation and purification

CLC Number:

Q936

Lian-kui WEN. Isolation and Purification of Key Tartaric Acid Degrading Enzyme from Aspergillus niger F1[J]. FOOD SCIENCE, 2012, 33(15): 235-238.

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