Protective Effect of Chrysalis Oil against Three Different Types of Liver Injury in Mice

Abstract

Abstract: Objective: To investigate the protective effect of chrysalis oil against three different types of liver injury in mice. Methods: A total of 150 male ICR mice were equally randomized into 3 groups by weight: group a (acute alcoholic hepatic injury group), group b (immunological liver injury group), and group c (D-galactosamine-induced liver injury group). Then the mice in each group were further randomly assigned into 5 subgroups: normal control, evening promise oil, liver injury control, low and high-dose chrysalis oil subgroups. After administration for 7 days, the mice in each group (except mice in the normal control subgroup) were treated as follows: the mice in group a were given 50% ethanol (12 mL/kg) to establish a mouse model of alcoholic liver injury; the mice in group b were intravenously injected with Bacille de Calmette Guerin (BCG) + lipidpolysaccharide (LPS) to induce a mouse model of immune liver injury; the mice in group c were intraperitoneally injected with D-GAL (600 mg/kg) to induce a mouse model of acute liver injury. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and liver malondialdehyde (MDA) and glutathione (GSH) content were measured 24 hours later. Results: The liver and spleen indexes, serum ALT and AST activities, and MDA and GSH content in the liver of mice from the chrysalis oil groups were significantly different to those of the liver injury control group (P ＜ 0.05). The liver and spleen indexes were significantly correlated with ALT and AST activities in the blood and MDA and GSH levels in the liver (P ＜ 0.05). Conclusion: Chrysalis oil has a significant protective effect against ethanol induced acute liver injury, BCG and LPS induced liver injury and D-GAL induced liver injury in mice. Its protective effect against alcoholic liver injury and BCG+LPS induced liver injury is greater than that of evening primrose oil. The mechanism may be related to its property to reduce lipid peroxidation in the body and its ability to prevent liver cells from necrosis.

Key words: chrysalis oil, polyunsaturated fatty acids, liver injury, lipid peroxidation

CLC Number:

References

[1] 沈洪,张蓓.急性肝损伤研究进展[J].现代中西医结合杂志,2009,18(18):2211-2213.
[2] 范建高,丁晓东.酒精性肝炎的治疗现状[J].中华肝脏病杂志,2003,11(11): 703-704.
[3] 陈慧敏,高南南.中药抗氧化作用治疗酒精性肝损伤的研究进展[J].中华中医药杂志,2009,24(7):912-914.
[4] Donohue TM. Autophagy and ethanol-induced liver injury[J].World Journal of Gastroenterology,2009,15(10):1178-1185.
[5] Ramírez-Farías C, Madrigal-Santillán E, Gutiérrez-Salinas J, et al. Protective effect of some
vitamins against the toxic action of ethanol on liver regeneration induced by partial
hepatectomy in rats[J].World Journal of Gastroenterology,2008,14(6):899-907.
[6] 宋燕青,邓树海,隋志义等.蚕蛹药用成分及其提取工艺研究概况[J].中国生化药物杂志,2006,27(5):306-309.
[7] Wang C, Harris WS, Chung M, et al. N-3 Fatty acids from fish or fish oil supplement, but not
α-linolenic acid, benefit cardiovascular disease outcomes in primary-and
secondary-prevention studies: a systematic review[J]．Am J Clin Nutr,2006,84:5?17．
[8] Lovegrive JA, Lovegrive SS, Lesauvade SV, et al. Moderate fish-oil supplementation reverses
low-platelet, long-chain n-3 polyunsaturated fatty acid status and reduces plasma
triacylglycerol concentration in British Indo-Asians [J]. Am J Clin Nutr, 2004, 79:974?982.
[9] Geleijnse JM, Giltay EJ, Grobbee DE, et al. Blood pressure response to fish oil supplementation: metaregression analysis of randomized trial[J]. Hypertens, 2002, 20:1493?1499.
[10] 何自立，陈伟平，单巍．蚕蛹油对大鼠非酒精性脂肪肝形成的影响[J]．中国微生态学杂志，2007，19（6）：483-485．
[11] 薛川松,杨钦河,刘淑娟等.降脂宁肝胶囊对D-半乳糖胺致小鼠急性肝损伤的保护作用[J].时珍国医国药,2009,20(02):295-296.
[12] Sugiyama A, Sato A, Shimizu H,et al. Pegylated lactoferrin enhances its hepatoprotective effects on acute liver injury induced by dgalactosamine and lipopolysaccharide in rats[J]. The Journal of Veterinary Medical Science, 2010, 72(2): 173-180
[13] 郑荣梁，黄中洋．自由基生物学[M]．3版．北京：高等教育出版社，2007：95—108．
[14] Auza N J， William G D ，M ichael J M ． Diagnosis and treatment of copper toxicosis in ruminants[J]．Journal of the American Veterinary Medical Association，1999，214：1 624—1 628．
[15] Wu Y，Ij L，Wen T，et a1．Protective efects of Echinacoside on carbon tetmchlofide induced hepatotoxicity in rats[J]．Toxicology，2007，232(1)：50—56．
[16] Samar Basu．Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients[J]．Toxicology，2003，189(2)：113—127．
[17] Mehmetcik G，Ozdemirler G，Kocak Toker N，et a1．Efect of pretreatment with artichoke extract on carbon tetrachloride— induced liver injury and oxidative stress[J]．Experimental and Toxicologic Pathology，2008，60(1)：475—480．
[18] 刘海军,苏超,付中华.也谈蚕蛹油的开发利用[J].北方蚕业,2008, 29(4):40-41.
[19] 魏兆军, 廖爱美, 胡海梅等. 蚕蛹油的成分、营养价值及提取工艺研究进展[J]. 安徽农业科学,2007(30):9699-9700,9711.
[20] 邱林立,罗德生,许娟,潘代,黄佳,卢淑梅,吴卉文.乌索酸对小鼠酒精性肝损伤的保护作用[J]. 咸宁学院学报（医学版）,2009,23(2):97-99
[21] 赵敏,池莉平,王凤岩等.小鼠急性酒精性肝损伤模型的建立及应用[J].华南预防医学,2005,31(1):14-17.
[22] Shepard BD, Tuma PL.Alcohol-induced alterations of the hepatocyte cytoskeleton[J]. World Journal of Gastroenterology, 2010, 6(11): 1358–1365.
[23] 雷红伟，高铁祥，江南，等．复方解酒药抗乙醇脂质过氧化作用的研究[J]．湖北中医杂志，2007，29(8)：57．
[24] 吴丽萍,刘嵘,毕玉顺等.复肝灵颗粒对鼠免疫性肝损伤的保护作用[J].山东大学学报（医学版）,2008,3(3):237-240.
[25] 曹家麟,吴春明,朱小区等.二草清肝汤对大鼠免疫性肝损伤的保护作用及其机制[J].临床医学,2008,28(3):109-111.
[26] 陈雪龙．实验性肝损伤动物模型的研究进展[J]．实验动物科学，2008．15(3)：47．