Isolation, Identification and Biological Characterization of a Strain of Alicyclobacillus from Orchard Soil in Shaanxi

Abstract

Abstract: The aim of this study was to isolate Alicyclobacillus from orchard soil collected from apple farms in Shaanxi province, China and to study the distribution of Alicyclobacillus. A strain of Alicyclobacillus was isolated by using a YSG medium acidified with sulphuric acid. The morphology, cultural characteristics and physiological characteristics of the isolate were tested and the carbohydrate fermentation and enzyme profile were investigated by API ZYM and APY 50CHB System. The fatty acid composition was analyzed by GC. In addition, a phylogenetic tree was constructed by 16S rDNA sequence analysis. The strain of Alicyclobacillus was basically consistent with the reference stain Alicyclobacillus acidoterrestris DSM 3922T in morphology, and showed high similarity in enzyme profile, but great differences in carbohydrate fermentation performance. 16S rDNA sequence analysis indicated that both strains belonged to different species of the same genus. Moreover, the isolated strain shared a homology of 99% with the reference strain. In conclusion, the internationally recognized method for Alicyclobacillus isolation in combination with API System, fatty acid composition analysis and 16S rDNA sequence analysis permits rapid isolation and identification of Alicyclobacillus. As expected, Alicyclobacillus is distributed in orchard soil, which may pose a threat to the quality of fruit juice.

Key words: Alicyclobacillus acidoterrestris, API system, 16S rDNA, fatty acid, identification

CLC Number:

中图分类号: TS275.5

References

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