Composition Analysis in Ethanol Extracts of Propolis Samples from Northern China

Abstract

Abstract: The purpose of this study was to compare the contents of total phenols, total flavonoids and individual components in ethanol extracts of propolis samples collected from Northern China (Beijing and 10 provinces). The content of total flavonoids and total phenols were determined by NaNO2-Al(NO3)3 method and Folin-Ciocalteau method, respectively. Quantitative and qualitative HPLC analysis of phenols in propolis extracts was carried out by comparison with standard references for geographic cluster analysis of propolis samples. The results showed that the contents of total flavonoids and total phenols of propolis samples varied with geographic origin. The highest contents of caffeic acid, kaempferol, pinocembrin, chrysin, galangin and benzyl cinnamate were found in propolis samples from Henan province; propolis samples from Liaoning province were the richest in apigenin and isorhamnetin; propolis samples from Shandong province showed the highest content of rhamnetin.

Key words: propolis extract, HPLC, composition analysis

CLC Number:

S896

References

[1] 董捷，张红城，尹策，李春阳.蜂胶研究的最新进展[J].食品科学，2007，28(9):639-642.
[2] Chen C N, Weng M S, Wu C L, et al. Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cell by Taiwan Propolis Different Source [J]. Evidence-Based Complementary and Alternative Medicine, 2004, 1(2): 175-185.
[3] Yu Z G, Bao B L, Lu B Y, et al. Determination of ?avone C-glucosides in antioxidant of bamboo leaves (AOB) forti?ed foods by reversed-phase high-performance liquid chromatography with ultraviolet diode array detection [J]. Journal of Chromatography A, 2005, 1065: 177-185.
[4] Stephen M B, Carol H C W, Betty Y S, et al. Identi?cation of ?avone aglycones and glycosides in soybean pods by liquid chromatography–tandem mass spectrometry [J]. Journal of Chromatography A, 2003, 991: 61-68.
[5] Zhou J H, Li Y, Zhao J, et al. Geographical traceability of propolis by high-performance liquid-chromatography ?ngerprints [J]. Food Chemistry, 2008, 108: 749-759.
[6]张红城, 董捷, 李慧, 等. 六种蜂花粉多酚和黄酮类物质含量测定及抗氧化性的研究[J]. 食品科学, 2007, 28(9): 500-504.
[7]董捷, 张红城, 秦健, 等. 十种蜂花粉醇提物中总多酚和总黄酮含量测定[J]. 食品科学, 2008, 29(12): 246-249.
[8] Katarina H, Jozef L, Jozef C. Determination of organic acid in propolis by HPLC using two columns with an on-line SPE system [J]. Journal of Liquid Chromatography and Related Technologies, 2009, 32(1): 125-135.
[9]Ahn M R, Kumazawa S, Usui Y, et al. Antioxidant activity and constituents of propolis collected in various areas of China [J]. Food Chemistry, 2007, 101: 1383-1392.
[10]He N, Wang Z Y, Yang C X, et al. Isolation and identi?cation of polyphenolic compounds in longan pericarp [J]. Separation and Puri?cation Technology, 2009, 70: 219-224.
[11] 陈磊, 黄雪松. 高效液相色谱法同时检测黄酒中的5-羟甲基糠醛和9种多酚[J]. 分析化学研究简报, 2010, 38(1): 133-137.
[12]Wang D M, Lua J L, Miao A Q, et al. HPLC-DAD-ESI-MS/MS analysis of polyphenols and purine alkaloids in leaves of 22 tea cultivars in China [J]. Journal of Food Composition and Analysis, 2008, 21: 361-369.
[13] Garcia-Viguera C, Ferreres F, Tomas-Barberan F A. Study of Canadian propolis by GC-MS and HPLC [J]. Zeitschrift fur Naturforschung C: A Journal of Biosciences, 1993, 48(5-6): 731-735.
[14] Maciejewicz W, Daniewski M, Dzido T H, et al. GC-MS and HPLC analysis of phenolic acids extracted from propolis and from Populus nigra bud exudates [J]. Chemistry Analysis (Warsaw), 2002, 47(1): 21-30.
[15] Vardar-Unlu G, Silici S, Unlu M. Composition and in vitro antimicrobial activity of populus buds and polar-type proplis [J]. Chemistry and Materials Science, 2008, 24(7): 1011-1017.
[16] Bankova V, Popova M, Bogdanov S, et al. Chemical composition of European propolis: expected and unexpected results [J]. Zeitschrift fur Naturforschung C: A Journal of Biosciences, 2002, 57(5-6): 530-533.
[17] Volpi N, Bergonzini G. Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry [J]. Journal of Pharmaceutical and Biomedical Analysis, 2006, 42(3): 354-361.
[18]Medana C, Carbone F, Aigotti R, et al. Selective analysis of phenolic compounds in propolis by HPLC-MS/MS [J]. Phytochemical Analysis, 2008, 19(1):32-39.