Determination of Taurine in Porphyra yzoensis by High Performance Liquid Chromatography

Abstract

Abstract: An HPLC method was proposed to determine the content of taurine in Porphyra yzoensis. Taurine was subjected to pre-column derivatization with OPA. The separation was performed on a XDB C18 (150 mm × 4. 6 mm, 5 μm) using a mobile phase composed of methanol and pH 5.3 sodium acetate buffer (30:70) at a flow rate of 1 mL/min. Taurine was detected at a 315 nm wavelength. The column temperature was set at 30 ℃. Taurine was well separated with a good linear relationship in the range of 5.0－300.0μg/mL (R2 = 0.9991). The average recovery was 98.8% (n = 6) with a relative standard deviation of 1.8%. The taurine derivative was stable within 15 min. The limit of detection for taurine was 0.25μg/mL. The propose HPLC method was successfully applied to determine taurine in Porphyra yzoensis with a result of (1.04 ± 0.07)% (dry wt). This method was interference-free, simple, rapid, specific, sensitive, accurate and reliable.

Key words: high performance liquid chromatography, pre-column derivatization, taurine, Porphyra yzoensis

CLC Number:

TS201.7

References

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