Analysis of Fatty Acids in Rana chensinensis Eggs by Two Different Extraction Methods Combined with GC-MS

Abstract

Abstract: The egg oil of Rana chensinensis was extracted by Bligh-Dyer method or Soxhlet extraction method, methyl esterfied, and then analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 27 fatty acids were identified in the egg oil obtained by Bligh-Dyer method, including 16 unsaturated fatty acids (72.49%), such as arachidonic acid methyl ester (5.72%), eicosapentaenoic acid (5.72%), docosahexaenoic acid (5.02%), 9,12-octadecadienoic acid (8.89%), and 5,8,11,14-eicosatetraenoic acid (5.72%). Twelve fatty acids were identified by Soxhlet extraction method combined with GC-MS, comprising 6 unsaturated fatty acids (64.86%), such as 9-octadecenoic acid (21.99%), 5,8,11,14,17-eicosapentaenoic acid (7.58%) and 9,12-octadecadienoic acid (5.22%).

Key words: Rana chensinensis eggs, fatty acid, gas chromatography-mass spectrometry (GC-MS)

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Analysis of Fatty Acids in Rana chensinensis Eggs by Two Different Extraction Methods Combined with GC-MS

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