Synthesis and Identification of Artificial Antigen for Bensulfuron

Abstract

Abstract: In this study, a hapten was synthesized from methyl 2-[(sulfamoyl)methyl] benzoate and succinic anhydride. The synthetic hapten was identified by thin layer chromatography and 1H-NMR spectroscopy. An immune antigen (hapten-BSA) and a coating antigen (hapten-OVA) were prepared through separate conjugation of the hapten with BSA and OVA by mixed anhydride method. The conjugation ratios of the immune antigen and coating antigen were calculated to be 8:1 and 13:1 based on UV spectroscopic analysis, respectively, suggesting their good immunogenicity. Positive sera with a titer of 1:32000 were obtained by animal immunization trials. The IC50 and IC10 of antibody were determined by indirect ELISA assay to be 0.5032 mg/L and 0.0024 mg/L, respectively. Therefore, the synthesis of artificial antigen is successful.

Key words: bensulfuron, sulfonylurea herbicide, ELISA

CLC Number:

TS207.3

Wang Lei. Synthesis and Identification of Artificial Antigen for Bensulfuron[J]. FOOD SCIENCE, 2012, 33(19): 223-226.

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