FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (21): 16-19.

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Comparison of the Changes in Glyceollin Production in Different Types of Soybeans (Giycine max) Induced by Silver Nitrate

  

  • Received:2012-06-20 Revised:2012-09-24 Online:2012-11-15 Published:2012-11-09

Abstract: Objective: To compare the changes in silver nitrate-induced glyceollin production in three types of soybeans: yellow, green and black, each of which consisted of three cultivars. Methods: Glyceollin production in soybeans was induced by 0.01 mol/L silver nitrate solution and determined by HPLC. The chromatographic separation was performed on a C18 column (4.6 mm × 250 mm, 5 μm) using a mobile phase consisting of acetic acid solution (pH 3) and acetonitrile at a flow rate of 1 mL/min through gradient elution. Glyceollin was detected at 285 nm. Column temperature was set at 40 ℃. Results: Under the same extraction and determination conditions, silver nitrate-induced glyceollin production in soybeans decreased in the following order: green > black > yellow. Conclusion: The results obtained in this study can provide evidence for the selection of suitable soybean type for research and applications of glyceollins.

Key words: HPLC, glyceollin, quantification, Giycine max