Abstract: In this study, cbh (cellobiohydrolase)Ⅱgene from Trichoderma viride, 1611 bp in length and containing a signal peptide sequence, was cloned by PCR and inserted into the nmt1 promoter (Pnmt1) in Schizosaccharomyces pombe expression vector to obtain a recombinant plasmid pESP-2-cbh Ⅱ. The recombinant plasmid was transformed into S. pombe SP-Q01 by electroporation. S. pombe transformants carrying cbh Ⅱgene were successfully obtained as demonstrated by PCR and double enzyme digestion. The transformants were inductively cultured and total proteins were extracted from them and detected by SDS-PAGE to contain a protein band of approximately 81 kD. The maximum cellobiohydrolase activity of the fermentation supernatant of the S. pombe transformants was measured by DNS method to be 165 U/mL. The above results indicated that cbh Ⅱ gene was successfully expressed in S. pombe, which was controlled by Pnmt1 promoter and that the signal peptide sequence of the cbh Ⅱ gene was correctly recognized by S. pombe to produce extracellular expression products.

Key words: Trichoderma viride, cellulase, cbhⅡ gene, Schizosaccharomyces pombe, cellubiohydrolase activity