Optimization of Liquid Fermentation Conditions for Xylanase Production by a Actinomyces and Characterization of the Enzyme

Abstract

Abstract: In the current study, high-xylanase-producing strain was screened from different soil samples by transparent circle method, using xylan as the only carbon source in medium. The cultural condition for xylanase production by strain L10608 was optimized and the hydrolysis property of the enzyme was further investigated. The results indicated that the optimum fermentation medium contained a carbon source of water-insoluble xylan (80 mesh) of 25 g/L, compound nitrogen source of soya peptone of 25 g/L and yeast extract of 5 g/L, initial pH 6.0, cultural temperature 40 ℃, rotational speed of 200 r/min, surfactant polysorbate 80 of 4 g/L . Under the optimized condition, the enzyme activity reached 1074.8 U/mL. The xylanase utilized all birch wood xylan, beech wood xylan and oat xylan as the substrate, exhibiting that xylanase produced by L10608 was endo-xylanase with xylobiose and xylotriose as the major hydrates. These results showed that strain L10608 could hopefully be used for industrial production of functional xyloolygosaccharides.

Key words: actinomycetes, xylanase, liquid fermentation, optimize, xyloolygosaccharides

CLC Number:

TQ920.1

References

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