Screening for Cellulase Producing Edible Fungi and Optimization of Medium

Abstract

Abstract: The ability of six edible fungi, Russula delica Fr, Flammulina velutipe, Lentinus javanicus, Bamboo-sun, Russula lepida and Abalone mushroom, to produce cellulase were compared using the Congo red saucer rapid screening method. Meanwhile, the optimum carbon source and medium formula for cellulase production were screened and optimized. The result showed that Russula lepida produced the largest transparent rings of 20 mm in diameter, showing its high yield of cellulase. Among the four kinds of carbon source, CMC-Na, bean dregs, straw powder and glucose, the bean dregs promoted Russula lepida to produce cellulase. The optimized medium for cellulase production by Russula lepida was bean dregs 60 g/L, ammonium sulfate 2.0 g/L, Tween-80 1.5 mL/L, and pH 5.4.

Key words: edible fungi, cellulase, screening, medium

CLC Number:

Q939.99

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