Optimization of Homogenization Conditions for the Extraction of Proanthocyanidin from Ginkgo biloba Leaves Using Response Surface Methodology

Abstract

Abstract: This study deals with the optimization of homogenization conditions for the extraction of proanthocyanidin from Ginkgo biloba leaves using response surface methodology. The effects of extraction solvent, solid-to-solvent ratio, and homogenization speed and time on the extraction yield of proanthocyanidin was investigated by one-factor-at-a-time method. Further investigations were carried out to optimize the process parameters through response surface analysis of a mathematical model for the extraction yield of proanthocyanidin as their function built based on a Box-Behnken experimental design. The optimal conditions for extracting proanthocyanidin from Ginkgo biloba leaves were found to be homogenization at a rotary speed of 10500 r/min for 5.87 min with an absolute ethanol-to-Ginkgo biloba leaves of 1:23.8 (g/mL). Under these conditions, the extraction yield of proanthocyanidin was 2.21%.

Key words: Ginkgo biloba leaves, proanthocyanidin, homogenization, extraction, response surface methodology

CLC Number:

References

[1] 孙达旺. 植物单宁化学[M]. 北京: 中国林业出版社, 1992: 4-6.
[2] SANTOS-BUELGA C, SCALBERT A. Review: Proanthocyanidins and tannin-like compounds nature, occurrence, dietary intake and effects on nutrition and health [J]. Journal of the Science of Food and Agriculture, 2000, 80(7): 1 094-1117.
[3] 孙传范. 原花青素的研究进展[J]. 食品与机械, 2010, 26(4): 146-152.
[4] 吕丽爽. 天然抗氧化剂低聚原花青素的研究进展[J]. 食品科学, 2002, 23(2): 147-150
[5] 张小军, 夏春镗, 吴建铭, 等. 原花青素的资源研究[J]. 中药材, 2009, 32(7): 1154-1160.
[6] 廖素凤, 陈剑雄, 黄志伟, 等. 响应曲面分析法优化葡萄籽原花青素提取工艺的研究[J]. 热带作物学报, 2011, 32(3): 554-559.
[7] BEEK T A V, MONTORO P. Chemical analysis and quality control of ginkgo biloba leaves, extracts, and phytopharmaceuticals[J]. Journal of Chromatography A, 2009, 1216: 2002-2032.
[8] 李超, 王卫东. 原花青素提取方法的研究进展. 粮油加工[J]. 2009, 17(9): 145-148.
[9] 祖元刚, 赵春建, 李春英, 等. 鲜法匀浆萃取烟叶中茄尼醇的研究[J]. 高校化学工程学报, 2005, 19(6): 757-761.
[10] SEZGINTBRKM K D, INCKAYA E. An amperometric inhibitor biosensor for the determination of reduced glutathione (GSH) without any derivatization in some p lants[J]. Biosensors and Bioelectronics, 2004, 19 (8): 835- 841.
[11] HOLFORD K C, EDWARS K A, BENDENA W G, et al. Purification and characterization of a mandibular organ protein from the American lobster, Homarus americanus: a putative farnes acid o2methyltransferase [J]. Insect Biochemistry and Molecular Biology, 2004, 34(8): 785-798.
[12] 张琳, 杨磊, 贾佳. 匀浆法提取长春花中长春碱、文多灵和长春质碱[J]，高校化学工程学报, 1996, 10(3): 258-263.
[13] 郑永丽, 李梦青. 原花色素的含量测定研究[J]. 广州化工, 2010, 38(5): 197-203.
[14] 姚开, 何强, 吕远平. 葡萄籽中原花青素含量不同测定方法比较[J]. 化学研究与应用，2002, 14(2): 230-232.
[15] 李春阳, 许时婴, 王璋. 香草醛-盐酸法测定葡萄籽、梗中原花青素含量的研究[J]. 食品科学, 2004, 25(2): 157-161.
[16] 徐向宏, 何明珠. 试验设计与Design-Expert、SPASS [M]. 北京:科学出版社, 2010: 5-10.
[17] CHIN L H, HAMEED B H, AHMAD A L. Process Optimization for Biodiesel Production from Waste Cooking Palm Oil (Elaeis guineensis) Using Response Surface Methodology. Energy & Fuels, 2009, 23 (2): 1040-1044.
[18] 董发明, 白喜婷. 响应面法优化超声提取杜仲雄花中黄酮类化合物的工艺参数[J]. 食品科学, 2008, 29(8): 227-231.