Genetic Diversity and Phylogeny of Lactic Acid Bacterial Strains Isolated from Yak Yoghurt in Western Sichuan Platea

doi:10.7506/spkx1002-6630-201205037

Abstract

Abstract: The genetic diversity and the phylogeny of 81 lactic acid bacterial strains isolated from yak yogurt samples collected from Western Sichuan Plateau were studied by using 16S rDNA-PCR-RFLP, ISR-PCR-RFLP techniques, and 16S rDNA gene sequence analysis. The results showed totally 23 16S rDNA genotypes were detected and the tested strains could be divided into four taxa on the basis of 61% similarity, and 16 taxa on the basis of 81% similarity. Sequence analysis of 16S rDNA genes showed that genus Lactobacillus and genus Enterococcus were two major genera in all tested strains. ISR-PCR-RFLP analysis also revealed 19 ISR genotypes, and the tested strains could be divided into four taxa on the basis of 60% similarity, and 14 taxa on the basis of 80% similarity.

Key words: yak yoghurt, lactic acid bacteria, similarity level, phylogeny

CLC Number:

TS252.1

TIAN Hong，PU Biao，ZHANG Xiao-ping. Genetic Diversity and Phylogeny of Lactic Acid Bacterial Strains Isolated from Yak Yoghurt in Western Sichuan Platea[J]. FOOD SCIENCE, 2012, 33(5): 170-176.

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Genetic Diversity and Phylogeny of Lactic Acid Bacterial Strains Isolated from Yak Yoghurt in Western Sichuan Platea

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