FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 327-330.doi: 10.7506/spkx1002-6630-201310072

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Effect of Vacuum Air Current Technique for Plant cell Wall Breakdown (VAPB) on the Extraction of Active Ingredients in Mulberry Leaves

SUN Chang-bo,SHI Lei-ling,TU Jian-fei,HAN Dan-dan,ZHANG Qing-qing,ZHANG Jing   

  1. 1. College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China;
    2. Development College, Jilin Agricultural University, Changchun 130600, China;
    3. Xinjiang Institute of Chinese Materia Medicao and Ethical Materia Medica, Ürümqi 830002, China
  • Received:2012-04-11 Revised:2013-03-22 Online:2013-05-25 Published:2013-05-07
  • Contact: Jing ZHANG E-mail:zhjing0701@163.com

Abstract:

Objective: To investigate the effect of VAPB on the extraction efficacy of alkaloids, flavonoids and
polysaccharides in mulberry leaves. Methods: The contents of alkaloids, flavonoids and polysaccharides in mulberry leaves
with damaged cell wall and intact wall were determined and compared by HPLC or spectrophotometric methods. Results:
The contents of alkaloids, flavonoids and polysaccharides in mulberry leaf samples with damaged cell wall were higher than
those with intact cell wall, which were 74%, 58% and 48% respectively. Conclusions: Pretreatment of mulberry leaves by
VAPB may significantly increase the solubility of the active components in mulberry leaves.

Key words: mulberry leaves, vacuum air current for plant cell wall breakdown, alkaloid, flavonoid, polysaccharide, extraction

CLC Number: