Flurescence Polarization Immunoassay for the Detection of Salbutamol

doi:10.7506/spkx1002-6630-201316028

FOOD SCIENCE

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Flurescence Polarization Immunoassay for the Detection of Salbutamol

YUAN Li-peng，YANG Jin-yi，XU Zhen-lin，SUN Yuan-ming，WU Cui-hua，LEI Hong-tao

1. Guangdong AIB Polytechnic College, Guangzhou 510507, China；2. Key Risk Assessment Laboratory of Agricultural Product Preservation, Ministry of Agriculture, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China

Online:2013-08-25 Published:2013-09-03

Abstract

Abstract:

A fluorescence polarization immunoassay (FPIA) was presented to detect salbutamol. Fluorescence-labeled
salbutamol tracer (SAL-HDF) was synthesized via the reaction of salbutamol-succinate (SAL) with fluoresceinthiocarbamyl
ethylendiamine (HDF). Several parameters that might affect the performance of FPIA such as SAL-HDF and anti-salbutamol
antibody concentration were optimized. Under the optimum conditions, the limit of detection was 72.36 ng/mL, the IC50 was
975.18 ng/mL, and the linear range of the developed FPIA was 178.08–4871.15 ng/mL. This method can provide a useful
reference to develop a salbutamol FPIA test kit.

Key words: fluorescence polarization immunoassay, tracer, salbutamol

YUAN Li-peng，YANG Jin-yi，XU Zhen-lin，SUN Yuan-ming，WU Cui-hua，LEI Hong-tao. Flurescence Polarization Immunoassay for the Detection of Salbutamol[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316028.

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Flurescence Polarization Immunoassay for the Detection of Salbutamol

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