Abstract:

A new method to determine microamounts of selenium in the seeds of Lens culinaris and Vigna minima was
established using resonance scattering light quenching. In pH 3.500 B-R buffer solution, selenium (Ⅳ) reacted with a certain
amount of I－ to produce an equal molar amount of I3－, which further reacted with rhodamine 6G to form an ion association
complex causing linear quenching of resonance scattering light at 592.2 nm. The results showed good linear relationship
between resonance scattering light relative quenching intensity ΔI and Se(Ⅳ) concentration in the range of 0–60 μg/L under
optimized conditions. The detection limit of the method was 1.710 μg/L. The precision (RSD) for 7 replicate measurements
of selenium in the seeds of Lens culinaris and Vigna minima was 3.9% and 1.9%, respectively, and the average spiked
recovery was 95.56% and 96.80%, respectively. These results were satisfactory.

Key words: lentils (Lens culinaris), Vigna minima, selenium, resonance scattering light quenching method