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Simultaneous Determination of Tau-Fluvalinate, Triadimenol and Coumaphos Residues in Royal Jelly Using High Performance Liquid Chromatography Tandem Mass Spectrometry

ZHOU Ping，LI Ying-hua，HU Fu-liang，ZHAO Huan，WU Jie，LIANG Shi-jun   

  1. 1. Hangzhou Beewords Apiculture Group Co. Ltd., Tonglu 311500, China；2. Zhejiang Economic and Trade Polytechnic, Hangzhou 310018, China；3. College of Animal Science, Zhejiang University, Hangzhou 310029, China；4. Measurement Center of Tonglu County, Hangzhou 311500, China
  • Online:2013-09-25 Published:2013-09-27

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Abstract:

A method for the determination and quantification of tau-fluvalinate, triadimenol and coumaphos in royal jelly was
established by HPLC-MS-MS. Tau-fluvalinate, triadimenol and coumaphos residues were extracted by acetonitrile under alkaline
condition, and clean-up of the extracts was carried out using HLB solid phase extraction (SPE) column. Quantification was conducted by
HPLC-MS-MS coupled with multiple reaction-monitoring (MRM) system using electron spray ionization source in an external standard
mode. The detection limits for coumaphos, triadimenol and tau-fluvalinate residues were 2, 1 μg/kg and 1 μg/kg, respectively. The
recovery rates of tau-fluvalinate, triadimenol and coumaphos residues at three spiked levels (5, 10 μg/kg and 50 μg/kg) in different samples
were in the range of 78.9%–102.1% with relative standard deviation of 2.1%–13.6%. The linear range of detection was 5–100 μg/kg (r ＞
0.9940). This method can be successfully applied to the qualitative and quantitative analysis of royal jelly samples.

Key words: royal jelly, tau-fluvalinate, triadimenol, coumaphos