Combined Mutation Screening of High-activity Saccharomyces cerevisiae Strain Capable of Producing Metallothionein

doi:10.7506/spkx1002-6630-201319053

Abstract

Abstract:

Saccharomyces cerevisiae 31206 was mutagenized by means of combined treatment with UV light, microwave and NTG.
After primary and secondary screening, a high-activity strain capable of producing metallothionein was obtained. Total protein content
was determined by Coomassie brilliant blue G-250 method, metallothionein content by ELISA, and sulfhydryl activity by DTNB
method. The total protein content, metallothionein content and sulfhydryl activity of the mutant strain were 170.2 mg/g cells, 163.4 ng/L
and 0.146 μmol, respectively, whereas those of the parental strain were 44.6 mg/g cells, 38.3 ng/L and 0.029 μmol, respectively.

Key words: Saccharomyces cerevisiae, combined mutation, screening, metallothionein

CLC Number:

F407.82

MIAO Lan-lan，ZHANG Dong-jie*，WANG Ying. Combined Mutation Screening of High-activity Saccharomyces cerevisiae Strain Capable of Producing Metallothionein[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201319053.

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Combined Mutation Screening of High-activity Saccharomyces cerevisiae Strain Capable of Producing Metallothionein

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