Abstract:

After being subjected to simultaneous treatment with a combination of enzymatic hydrolysis and extrusion, corn
four was saccharified. The effects of saccharification time, pH, glycosidase amount and liquid/solid ratio on saccharification
efficiency were investigated by measuring the dextrose equivalent (DE) of saccharified mash. Qualitative and quantitative
analysis of saccharified corn starch was performed by HPLC. The saccharification process was optimized by response
surface methodology to be 3.5 h at 60 ℃ using 280 U/g of glycosidase with a liquid/solid ratio of 4:1 (mL/g) at initial pH
5.0. Under these conditions, the yield of starch sugars was 106.39% and the DE value was 46.12%, in which the contents of
glucose, maltose and maltotriose were 31.87%, 33.47% and 12.91%, respectively.

Key words: extrusion/enzymolysis, combined treatment, saccharification, high performance liquid chromatography