A Polymerase Chain Reaction Method for Rapid Detection of Duck-Derived Materials in Meat Products

doi:10.7506/spkx1002-6630-201410030

Abstract

Abstract:

This report describes a rapid polymerase chain reaction (PCR) method to detect duck-derived DNA by using
primers corresponding to the duck COⅢ gene. The method was duck specific, free from interference from lumb DNA (50
mg/kg). Its sensitivity was sufficient to detect as low as 0.1 μg/kg duck-derived materials. It can provide a rapid and accurate
way to identify duck-derived materials adulteration in meat products without interference of mutton DNA.

Key words: duck-derived materials, cytochrome oxidase Ⅲ (COⅢ ) gene, polymerase chain reaction (PCR), detection

CLC Number:

S853.74

SHI Yan-yu1, LIU Jin-hua2, WANG Ying1, BING Wei1, HUA Lei1, LIU Xiao-hui1, WANG Ying1, ZHOU Liang1, WANG Xiao1. A Polymerase Chain Reaction Method for Rapid Detection of Duck-Derived Materials in Meat Products[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201410030.

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A Polymerase Chain Reaction Method for Rapid Detection of Duck-Derived Materials in Meat Products

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