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Using a Surface Plasmon Resonance Sensor for the Detection of Bacillus thuringiensis Cry2A Protein

CAI Miao1, HUANG Xin2, YUE Xi-qing1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: YUE Xi-qing

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Abstract:

Purpose: To establish a method to detect Bacillus thuringiensis (Bt) Cry2A protein by surface plasmon resonance
(SPR) sensor. Methods: By using SPR based on interaction between biomolecules, the monoclonal antibodies specific to
the Cry2A protein were modified on the gold surface for the detection of this protein. Results: The detection limit of Cry2A
protein was 10 ng/mL by this method without cross-reaction with Cry1Ac or Cry2Ab protein. Conclusion: The SPR method
was simple, time-saving, sensitive, specific and applicable for the qualitative detection of Bt Cry2A protein.

Key words: surface plasmon resonance sensor, Cry2A protein, Bacillus thuringiensis, genetically modified plant

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