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Effect of γ-Oryzanol on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in Macrophages

LIU Yuanjin1, TIAN Yuanyuan1, LIU Bo1, LI Xinhua2, CHEN Yaya1, ZENG Linna1, YANG Tao1, LIN Qinlu1, LUO Feijun1,*   

  1. 1. National Engineering Laboratory for Deep Processing of Rice and Byproducts, College of Food Science and Engineering,Central South University of Forestry and Technology, Changsha 410004, China;2. Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China
  • Online:2015-10-15 Published:2015-10-20

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Abstract:

In this study, we used macrophage models of lipopolysaccharide (LPS)-stimulated inflammation to evaluate the
effect of γ-oryzanol on the expression of inflammatory factors. After exposing RAW264.7 cells to different concentrations
of γ-oryzanol and LPS, we analyzed the contents of IL-1β, TNF-α, IL-6 and NO2-/NO3- (NO) in the macrophage culture
medium and found that γ-oryzanol could inhibit the secretion of inflammatory factors. Real-time quantitative PCR was
employed to analyzed mRNA expression levels of IL-1β, TNF-α, IL-6 and iNOS, and it was found that inflammatory gene
transcriptions were inhibited by γ-oryzanol. Western blotting analysis further confirmed that γ-oryzanol could decrease the
expression of IL-1β, TNF-α, IL-6 and iNOS protein. Our data showed that γ-oryzanol can significantly inhibit the expression
and secretion of inflammatory factors such as IL-1β, TNF-α, IL-6 and NO.

Key words: γ-oryzanol, inflammation, inflammatory factor, rice bran

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