Abstract:

The production process of polypeptide and soluble dietary fiber (SDF) from soybean meal with solid-state
fermentation by Mucor was investigated and optimized with response surface methodology. After optimization, the optimal
process conditions were determined as 20 g of soybean meal in a 250 mL shake flask with the addition of 3.0% xylose (g/g
soybean meal), 0.3% KH2PO4, 0.1% Tween-80 and 0.39% CaCl2 as the nutrient supplements, initial water content of 52.7%,
pH 5.68, fermentation temperature of 27 ℃ and fermentation time of 3 days. Under these conditions, the yield of polypeptide
and SDF could reach 16.1% and 18.5%, respectively. The experiment results show that the production process is feasible.

Key words: Mucor, soybean meal, solid-state fermentation, polypeptide, soluble dietary fiber