Abstract:

In this paper, the phospholipids in the brain of channel catfish were extracted and purified. A high-performance
liquid chromatography equipped with an evaporative light scattering detector (HPLC-ELSD) was used to analyze the
phospholipid components. The results indicated that the content of phosphatidylcholine (PC) was the highest among the
detected phospholipids phospholipids in channel catfish brain, which accounted for 45.73% of the total phospholipids,
followed by phosphatidylethanolamine (PE), which accounted for 17.39%. The proportions of phosphatidylserine (PS)
and proporphosphatidylinositol (PI) in relative to the total phospholipids were 5.01% and 1.20%, respectively. Under optimized
experimental conditions, the respective peak areas were finely ?tted to a linear model within the indicated range, and each class of
phospholipids was separated well. The method also can be applicable for the determination of phospholipids in other samples.

Key words: channel catfish brain, phospholipids, high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD), quantitative analysis