DNA Extraction for RAPD Analysis of Dried Tremella fuciformis

doi:10.7506/spkx1002-6630-201504033

FOOD SCIENCE

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DNA Extraction for RAPD Analysis of Dried Tremella fuciformis

WANG Fanhua1, MA Wutiao2, TAN Qingyun1, LIU Weixian2, SUN Tian2, DU Bing3, YANG Gongming3, YI Jicai1,*

1. Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Insititutions,
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China; 2. Infinitus (China) Co. Ltd.,
Jiangmen 529156, China; 3. College of Food Science, South China Agricultural University, Guangzhou 510642, China

Online:2015-02-25 Published:2015-02-16
Contact: YI Jicai

Abstract

Abstract:

It is difficult to extract genomic DNA from Tremella fuciformis due to the disturbance of abundant
polysaccharides and proteins. In this paper, a method is reported to directly extract DNA from dried Tremella fuciformis
without spore separation or mycelium culture procedures which enables one to achieve high-quality DNA in a high yield for
RAPD (random amplified polymorphic DNA) analysis. The RAPD results indicated the presence of DNA polymorphisms
in the tested samples. The results of this study will contribute to DNA extraction and variety identification of dried Tremella
fuciformis in food analysis and detection.

Key words: dried Tremella fuciformis, DNA extraction, random amplified polymorphic (RAPD) DNA, variety identification

CLC Number:

WANG Fanhua1, MA Wutiao2, TAN Qingyun1, LIU Weixian2, SUN Tian2, DU Bing3, YANG Gongming3, YI Jicai1,*. DNA Extraction for RAPD Analysis of Dried Tremella fuciformis[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201504033.

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DNA Extraction for RAPD Analysis of Dried Tremella fuciformis

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