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Determination of Different Synthetic Dyes in Ice-Cream by HighP erformance Capillary Electrophoresis with UV Detection

GUO Fangfang1, FENG Feng1,2,*, BAI Yunfeng2, CHEN Zezhong2, LIU Lizhen2, LI Rong2, ZHOU Gao2   

  1. 1. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
    2. School of Chemical and Environmental Engineering, Shanxi Datong University, Datong 037009, China
  • Online:2015-04-25 Published:2015-04-28
  • Contact: FENG Feng

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Abstract:

An efficient high performance capillary electrophoresis (HPCE) method with ultraviolet detector has been
successfully developed for the determination of brilliant blue, sunset yellow, amaranth, ponceau 4R and tartrazine in icecream.
The running buffer was composed of 10 mmol/L Na2B4O7 and 5 mmol/L NaH2PO4 containing 0.7 mmol/L betacyclodextrin
at pH 11.5. The detection wavelength was set at 214 nm and applied voltage 25 kV. Under these conditions, the
linear range was from 10 to 1 000 μg/mL with correlation coefficients between 0.997 5 and 0.999 9 and the limit of detection
(LOD) varied from 1.2 to 8.6 μg/mL (RSN = 3). The average recoveries (n = 3) were in the range from 93.8% to 108.5%, with
a relative standard deviation (RSD) lower than 4.19%. This method is convenient, accurate and suitable for the simultaneous
detection of multiple synthetic dyes.

Key words: high performance capillary electrophoresis, synthetic dyes, ice-cream

CLC Number: