Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance

doi:10.7506/spkx1002-6630-201508037

Abstract

Abstract:

Escherichia coli O157:H7 (E. coli O157:H7) was detected by a sandwich method with Au nanoparticles (AuNPs)
of different sizes labeled E. coli O157:H7 polyclonal antibody (PAb) as the second antibody and PAb immobilized on the
sensor surface as the first antibody using a dual-channel surface plasmon resonance (SPR) sensor. The abilities of second
antibodies labeled with AuNPs of three different sizes to enhance SPR signal were compared. As a result, the optimal AuNPs
size was found to be 17.79 nm and the minimum detectable concentration of E. coli O157:H7 was 10 CFU/mL.

Key words: AuNPs, E. coli O157:H7, surface plasmon resonance (SPR), labeling, different particle sizes

CLC Number:

S951.42

YANG Yang, LI Rongzhuo, MAO Lugang, LIU Xia*. Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201508037.

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Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance

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