FOOD SCIENCE

• Component Analysis • Previous Articles     Next Articles

Analysis of Nucleosides and Nucleobases of Polygonum multiflorum by Stepwise Regression Analysis

LUO Yiyuan1, LIU Juanxiu1, LIU Xunhong1,*, LAN Caiwu2, XU Li3, HOU Ya1, MA Yang1   

  1. 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;
    2. Research and Development Department, Guizhou Chang Hao Chinese Medicine Co. Ltd., Kaili 556000, China;
    3. Department of Chemistry, Yangzhou Institute for Drug Control, Yangzhou 225000, China
  • Online:2016-01-25 Published:2016-01-15
  • Contact: LIU Xunhong

RichHTML

3

PDF (PC)

165

Abstract:

This study analyzed 10 nucleosides and nucleobases in Polygonum multiflorum Radix and explored their effects
on the total content of nucleosides. Ten nucleoside and nucleobases in Polygonum multiflorum Radix were simultaneously
determined by ultra-performance liquid chromatography-tandem triple-quadrupole linear ion trap mass spectrometry (QTRAP
UPLC-MS/MS). The optimal equation describing total nucleoside content as a function of 10 nucleoside and nucleobases
was established by stepwise regression analysis. The results showed that the contents of uridine, adenine, guanosine and
cytidine were higher in Polygoni multiflori Radix. Stepwise regression analysis showed that uridine, cytidine and thymidine
had the most significant effects on the total content of nucleoside. The contents of uridine, cytidine and thymidine can be
used as an indicator the quality of for Polygonum multiflorum Radix based on total nucleoside, and provide a scientific basis
for constructing multiindicator quality evaluation system for Polygonum multiflorum Radix.

Key words: Polygoni multiflori Radix, ultra-performance liquid chromatography-tandem triple-quadrupole linear ion trap mass spectrometry (QTRAP UPLC-MS/MS), stepwise regression analysis, nucleosides, nucleobases

CLC Number: