Abstract: In this study, 3% konjac oligosaccharide was added to a high-fat diet to investigate the effect of konjac oligosaccharide on body mass, liver index, lipids and high-fat diet induced oxidative stress in mice. Reverse transcription-polymerase chain reaction (RT-PCR) was adopted to measure gene expression levels to explore the underlying mechanism. Male Kunming mice were randomly divided into normal diet, high-fat diet and oligosaccharide intervention groups. The results showed that the dietary addition of konjac oligosaccharide significantly reduced body mass and liver mass, improved blood lipid profile, and inhibited the generation of malondialdehyde in mice, which may be related to its ability to improve antioxidant capacity in the body. In addition, konjac oligosaccharide could significantly up-regulate the mRNA expression of carnitine palmityl transferase (CPT), thereby accelerating lipid decomposition and metabolism. It could also significantly up-regulate the mRNA expression of low density lipoprotein receptor (LDL-R) and reduce plasma low density lipoprotein cholesterol level. In summary, these results demonstrated that konjac oligosaccharide can effectively reduce the health risks caused by obesity.

Key words: konjac oligosaccharide, high-fat diet, blood lipid, oxidative stress