Abstract: Objective: To evaluate the inhibitory effect of konjac mannooligosaccharides (KMOS) on obesity and chronic inflammation in high-fat diet (HFD)-induced obese mice and to decipher the underlying molecular mechanisms. Methods: Ten-week-old healthy male C57BL/6J mice were randomly divided into 7 groups. After 12 weeks of continuous feeding, body mass, body fat, blood lipids, and oxidative stress indicators were detected. The indicators associated with lipid metabolism and their gene expression in adipose and liver tissues were analyzed. Results: KMOS effectively reduced body mass by 36.36% and body fat mass by 52.13%, improved blood lipid abnormality, and significantly inhibited the release of inflammatory factors and increased antioxidant enzyme activities (P < 0.05). Moreover, KMOS significantly reduced fat cell volume and inhibited lipid metabolism-related indicators and gene expressions in liver and adipose tissues (P < 0.05). Conclusion: KMOS can prevent obesity, and inhibit chronic inflammation and oxidative stress induced by obesity, which provides a scientific basis for the development of KMOS as functional dietary supplements against obesity and other chronic diseases.

Key words: konjac mannose oligosaccharide, obesity, anti-hyperlipidemia, high-fat diet, inflammation