Abstract:

In order to understand effects of pH value on glutathione (GSH) biosynthesis by Saccharomyces cerevisiae, a pHcontrol strategy was developed and applied in fed-batch production of GSH. Extracellular concentration of gultathione reached 47.66 mg/L when fermentation broth pH value was reduced to 1.5, and intracellular concentration of glutathione was 0.1% The addition of 0.1% SDS into fermentation broth revealed an inhibition effect on yeast growth. In contrast, sucrose ester, Tween- 80, DMSO did not exhibit effects on inhibition of yeast growth and secretion of GSH. On the other hand, yeast growth and secretion of GSH were obviously affected by Triton X-100. The higher concentration of Triton X-100 and the earlier addition resulted in more secretion of GSH.

Key words: glutathione, Saccharomyces cerevisiae, biosynthesis , Triton X-100