Abstract:

This study developed a specific high-performance liquid chromatography-electrospray ionization ion trap mass spectrometric (HPLC-ESI-MS/MS) method that allowed simultaneous determination of 15 herbicide multi-residues in grain samples. Residues of the herbicides were extracted from grains with acetonitrile, cleaned up using florisil-SPE, and then determined by HPLC-ESI-MS/MS. The method showed high degree of linearity (the correlation coefficients were 0.9834－0.9997) over the range of 0.01－10 mg/kg for the analytes. The limit of quantifications ranged from 0.002 to 0.07 mg/kg. The average recoveries for the herbicides in spiked samples were between 74.44% and 115.56% with a relative standard deviations from 0.37% to 13.79%. The method developed was applied to detect the targets in real samples, and several herbicide residues were identified.

Key words: herbicide, multi-residue analysis, grain