Real-time Fluorescent PCR Detection of Allergen in Sesame

doi:10.7506/spkx1002-6630-200912047

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 213-214.doi: 10.7506/spkx1002-6630-200912047

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Real-time Fluorescent PCR Detection of Allergen in Sesame

MA Li-dan1，CAO Ji-juan2，GAO Hai-yan3，BA Zhong-hua1

(1. Dandong Entry-Exit Inspection and Quarantine Bureu, Dandong 118000, China；2. Liaoning Entry-Exit Inspection and
Quarantine Bureu, Dalian 100161, China；3. College of Food, Henan Institute of Science and Technology, Xinxiang 453003, China)

Received:2008-09-09 Revised:2009-01-09 Online:2009-06-15 Published:2010-12-29
Contact: MA Li-dan1， E-mail:2212097@163.com

Abstract

Abstract:

This study aimed to develop a real-time fluorescent PCR assay for specific detection of allergen in sesame. Realtime PCR amplification was conducted to DNA fragments from 22 samples including white sesame and black sesame as well as other different samples using a primer pair and a probe specific for sesame DNA fragment. Both the black sesame and white sesame DNA fragments exhibited fluorescent signal, but others did not. Sensitivity analysis showed that 4.4×10-12g of sesame DNA with the dilution of 10-5 could be detected, and the quantitative formula was Ct = －3.472018l ln (initial copy number) + 18.851009, R2 = 0.993088, indicating that the primer pair and the probe have high sensitivity and amplification efficiency and fit with the requirements of trace detection.

Key words: sesame, real-time PCR, allergen detection

CLC Number:

R151.3

MA Li-dan1，CAO Ji-juan2，GAO Hai-yan3，BA Zhong-hua1. Real-time Fluorescent PCR Detection of Allergen in Sesame[J]. FOOD SCIENCE, 2009, 30(12): 213-214.

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Real-time Fluorescent PCR Detection of Allergen in Sesame

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