Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC

doi:10.7506/spkx1002-6630-200908053

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 238-240.doi: 10.7506/spkx1002-6630-200908053

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Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC

LIU Wen-hui1,2 XING Yu-xiu1,2 DONG Qi1 PI Li1 HU Feng-zu1,*

(1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2008-06-24 Revised:2008-12-06 Online:2009-04-15 Published:2010-12-29
Contact: HU Feng-zu1,* E-mail:hufz@nwipb.ac.cn

Abstract

Abstract:

The quantitative determination of inosinic acid and inosine was conducted by high performance liquid chromatography(HPLC)．The separation was carried out with analytical Kromasil C18 column (5 μm, 4.6 mm×250 mm), the mobile phase consisted of methanol and water (95:5, V/V), the flow rate was 1 ml/min and UV detection was performed at 254 nm. The results showed that there are good linear relationships between inosinic acid concentration and its HPLC peak area and between inosine concentration and its HPLC peak area in the range of 12.96 to 207.50 mg/L and 1.40 to 22.42 mg/ L, respectively. The average recovery rates were 97.98% and 98.26% for inosinic acid and inosine respectively．This method is fit for the quantitative analysis of inosinic acid and inosine in muscle of animals.

Key words: HPLC, inosinic acid, inosine, Tibetan sheep muscle

CLC Number:

TS207.3

LIU Wen-hui1,2 XING Yu-xiu1,2 DONG Qi1 PI Li1 HU Feng-zu1,*. Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC[J]. FOOD SCIENCE, 2009, 30(8): 238-240.

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Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC

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