Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor

doi:10.7506/spkx1002-6630-200902044

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (2 ): 201-203.doi: 10.7506/spkx1002-6630-200902044

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Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor

YUAN He-qing1，LI Ping1，LIU You-sheng2，ZHOU Chang-sheng2，CAO Ming2

(1. Changsha Environmental Protection College, Changsha 410004, China；
2. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China)

Received:2007-11-22 Revised:2008-03-22 Online:2009-01-15 Published:2010-12-29
Contact: YUAN He-qing1 E-mail:hqyuan@126.com

Abstract

Abstract:

Mycobacterium tuberculosis is the pathogenic microorganism of tuberculosis, and it could be infected by contaminated food. A piezoelectric bulk acoustic wave biosensor was used for rapid detection of M. tuberculos attenuated strain H37Ra in food samples. With the pool coefficient of 0.513 cm and the 10-fold diluted culture medium, it was found that the biosensor had a relatively high detection sensitivity, and a linear equation between cell concentrations and wave frequency was also constructed. It was also found that a pre-treatment with 2% NaOH solution could avoid the interference of mixed bacteria but prolong the detection period.

Key words: Mycobacterium tuberculos, piezoelectric bulk acoustic wave biosensor, food detection

CLC Number:

TS207.3

YUAN He-qing1，LI Ping1，LIU You-sheng2，ZHOU Chang-sheng2，CAO Ming2. Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor[J]. FOOD SCIENCE, 2009, 30(2 ): 201-203 https://doi.org/10.7506/spkx1002-6630-200902044

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Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor

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