Extraction of Flagellin from Listeria monocytogenes

doi:10.7506/spkx1002-6300-200923059

Abstract

Abstract:

In this study, the extraction of flagellin from Listeria monocytogenes was studied. Listeria monocytogenes IVb was cultured at 23 ℃ and 37 ℃, respectively. Stronger flagellin-producing capability was observed at 23 ℃ of culture condition. Flagellin was purified through ultra-centrifugation and ammonia sulfate precipitation after acid hydrolysis treatment of Listeria monocytogenes. Results from SDS-PAGE and electronic microscope revealed that ultra-centrifugation combined ammonia sulfate precipitation was an applicable method for flagellin extraction because this simple and time-saving method could provide flagellin with high purity and high yield.

Key words: Listeria monocytogenes, acid hydrolysis, flagellin, ultra-centrifugation

CLC Number:

TS207.3

CHEN Gang，LAI Wei-hua*，DUAN Xia. Extraction of Flagellin from Listeria monocytogenes[J]. FOOD SCIENCE, 2009, 30(23 ): 262-265.

[1]	SUN Hailei, DONG Pengcheng, MAO Yanwei, LIANG Rongrong, YANG Xiaoyin, ZHANG Yimin, ZHU Lixian, LUO Xin. A Review on Stress Response and Underlying Mechanisms in Listeria monocytogenes during Food Processing [J]. FOOD SCIENCE, 2021, 42(17): 317-324.
[2]	YUAN Ning, ZHANG Yunzhe, ZHANG Haijuan, YU Ze, LU Xin, ZHANG Wei. Detection of Listeria monocytogenes in Foods by Visual Saltatory Rolling Circle Amplification [J]. FOOD SCIENCE, 2021, 42(16): 239-245.
[3]	YANG Shiyi, LIN Wei, YANG Liyu, ZUO Cheng, LUO Qin. The Global Transcriptional Regulator CodY Promotes Oxidative Stress Resistance in Listeria monocytogenes [J]. FOOD SCIENCE, 2021, 42(14): 46-53.
[4]	SUN Linjun, ZHANG Hongzhi, FANG Taisong, WANG Yuan, LIU Yangtai, WANG Xiang, LI Hongmei, LI Daixi, DONG Qingli. A Review of the Biofilm Formation and Transfer of Listeria monocytogenes in the Food Environment and Prevention and Control Measures for It [J]. FOOD SCIENCE, 2021, 42(13): 289-299.
[5]	HUANG Ruijie, LI Mei, LIAO Anping, LAN Ping, ZHONG Lei, QIN Qin, LAN Lihong, WANG Xuejiao, WEI Liming, GAN Lanfang. Comparison of Acid Hydrolysis and Enzymatic Hydrolysis of Dextran [J]. FOOD SCIENCE, 2020, 41(6): 43-50.
[6]	TIAN Muyu, ZHANG Yimin, DONG Pengcheng, MAO Yanwei, LIANG Rongrong, ZHU Lixian, LUO Xin. The Mechanisms of Acid Tolerance Response of Salmonella and Listeria monocytogenes: A Review [J]. FOOD SCIENCE, 2019, 40(5): 316-322.
[7]	WU Xueyou, ZHU Yue, CHEN Zhengxing, JU Xingrong. Antibacterial Activity and Mechanism of Durancin GL against Listeria monocytogenes [J]. FOOD SCIENCE, 2019, 40(23): 73-78.
[8]	LIU Yunge, LUO Xin, DONG Pengcheng, ZHU Lixian, MAO Yanwei, LIANG Rongrong, YANG Xiaoyin, HAN Guangxing, ZHANG Yimin. Biofilm Formation and Control of Listeria monocytogenes Based on Quorum Sensing: A Review [J]. FOOD SCIENCE, 2019, 40(21): 303-310.
[9]	ZHENG Lan, MA Yaohong, MENG Qingjun, WANG Binglian, YANG Junhui, LIU Qing’ai, PENG Yao, HAN Fang. Preparation and Hydrolysis Characteristics of Mycelial Polysaccharide from Thelephora ganbajun Zang [J]. FOOD SCIENCE, 2019, 40(14): 312-322.
[10]	SUN Wanxia, JIN Yuqin, DAI Yingxiu, XIAO Jianwei, WANG Xiang, DONG Qingli. Application of Zero-Inflated Models in Quantitative Exposure Assessment of Listeria monocytogenes in Bulk Cooked Meat [J]. FOOD SCIENCE, 2019, 40(11): 49-54.
[11]	Sun Haitao,, Shao Xinru, QU Zhaoting, QIN Ting , MA Zhongsu. Preparation and Characterization of Corn Straw Nanocellulose [J]. FOOD SCIENCE, 2018, 39(8): 205-211.
[12]	NIU Ben1, MU Lili, ZHANG Zhaohuan, LIU Haiquan, PAN Yingjie, ZHAO Yong. Development of Predictive Models Based on qPCR for the Growth of Listeria monocytogenes and Vibrio parahaemolyticus Coexisting on Cooked Shrimps Stored at Low Temperatures [J]. FOOD SCIENCE, 2018, 39(23): 1-6.
[13]	JIANG Ronghua, DU Jianping, CUI Yang, ZHANG Chunyan, LIU Yangtai, ZHU Jianghui, WANG Xiang, LIU Qing, DONG Qingli. Quantitative Risk Assessment of Cross-Contamination of Listeria monocytogenes during Low-Temperature Emulsified Sausage Processing [J]. FOOD SCIENCE, 2018, 39(23): 134-141.
[14]	JIANG Ronghua, SU Liang, REN Pengcheng, SUN Linjun, WANG Xiang, LIU Qing, DONG Qingli. Suggestions concerning Limit Standard for Listeria monocytogenes during Low-Temperature Emulsified Sausage Processing [J]. FOOD SCIENCE, 2018, 39(22): 276-282.
[15]	ZHU Yahui, ZHU Hongri, WANG Jiaying, ZHANG Gongliang, HAO Hongshun, HOU Hongman. Virulence Gene Expression of Listeria monocytogenes Grown in Salmon Matrix [J]. FOOD SCIENCE, 2018, 39(2): 138-143.

Extraction of Flagellin from Listeria monocytogenes

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments