Abstract:

Clenbuterol (CL) was conjugated to horseradish peroxidase using glutaraldehyde at different ratios and a Micro DSC Ⅲ calorimeter was adopted to measure energy released during hydrolysis of α-naphthol by horseradish peroxidase labeled CL antigen with different conjugation ratios. A slight decrease was found in enzyme activity and the enzyme labeled antigen with lower conjugation degree showed a higher energy change under the same CL concentration. Under the same condition, the energy released during enzymatic reaction in 0.15 mol/L NaCl buffer was higher than that in 0.1 mol/L PBS buffer. In 0.15 mol/L NaCl buffer, the optimal concentration of α-naphthol was 20 mmol/L when the enzyme activity was 2 U.

Key words: clenbuterol, horseradish peroxidase labeled antigen, microcalorimetry